Construction and expression of mouse TRAF6-shRNA eukaryotic expressing plasmid
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منابع مشابه
Construction and Eukaryotic Expression of Recombinant Large Hepatitis Delta Antigen
Background: Hepatitis delta virus (HDV) is a subviral human pathogen that exploits host RNA editing activity to produce two essential forms of the sole viral protein, hepatitis delta antigen (HDAg). Editing at the amber/W site of HDV antigenomic RNA leads to the production of the large form (L-HDAg), which is required for RNA packaging. Methods: In this study, PCR-based site-directed mutagen...
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Background: DNA immunization with plasmid DNA encoding bacterial, viral, parasitic and tumor antigens has been reported to trigger protective immunity. Objective: To evaluate the use of a DNA immunization strategy for protection against anthrax, a plasmid was constructed. Methods: The partialsequence of protective antigen of Bacillus anthracis, amino acids 175-764, as a potent immunogenic targe...
متن کاملConstruction and direct immunization of eukaryotic expression plasmid encoding human soluble B lymphocyte stimulator.
B lymphocyte stimulator of the tumor-necrosis-factor family (BLyS) enhances B cell survival, a function indispensable to B cell maturation. This factor plays a crucial role in enhancing immune responses. Here, to study the primary immune effect of BLyS gene in animals, we constructed recombinant eukaryotic expression plasmid of the human soluble BLyS by using expression vector pcDNA3.1 (-). The...
متن کاملConstruction of Y376C-FGFR4 eukaryotic expression plasmid and its biological activity in HEK293 cell.
Jing Huang1, Liuxin Feng1, Hongli An2, and Tao Zhang1* School of Pharmaceutical Sciences, Xi’an Jiaotong University, Xi’an 710061, China Center for Translational Medicine, The First Affiliated Hospital, College of Medicine, Xi’an Jiaotong University, Xi’an 710061, China These authors are equal first author. *Correspondence address. Tel: þ86-29-82656788; Fax: þ86-29-82655451; E-mail: taozhang@ma...
متن کاملConstruction of the Recombinant Plasmid Expressing AID under the Control of Temperature-sensitive Promoter of Bacteriophage Lambda
Background and purpose: Activation-induced cytidine deaminase (AID) is a B-cell specific enzyme responsible for somatic hypermutation (SHM) and class switch recombination (CSR) of antibody genes within the B-cell follicle of peripheral lymphoid organs. Ectopic overexpression of the enzyme leads to mutations in non-B cells and Escherichia coli (E.coli) genes. However, induction of mutations in E...
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ژورنال
عنوان ژورنال: World Chinese Journal of Digestology
سال: 2009
ISSN: 2219-2859
DOI: 10.11569/wcjd.v17.i14.1406